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将先进的化学、光学和信息学技术相结合，实现了超凡的速度和数据质量、出色的通量和可扩展性。
Illumina Cell-Free DNA Prep with Enrichment是一种高度灵敏的文库制备解决方案，用于检测cfDNA样本中的低丰度突变。该工作流程包括用于纠错和降低假阳性率的唯一分子标记（UMI），从而准确、灵敏地检测低频突变。
Illumina cell-free DNA Prep with Enrichment
快速、灵活、可扩展的cfDNA文库制备试剂盒，用于高灵敏度突变检测
概述
快速、灵活的格式
Illumina cell-free DNA Prep with Enrichment与用户提供的因美纳或第三方供应商的富集寡核苷酸兼容，包括Integrated DNA Technologies（IDT）的单链DNA（ssDNA）和Twist Bioscience的双链DNA（dsDNA），具有更出色的内容可移植性。该试剂盒可用于55-2000 Kb（ssDNA）和70-2000 Kb（dsDNA）panel内容物，从而实现灵活的研究设计。可在约8.5-9.5小时内制备好可直接用于测序的文库，手动操作时间约为2.5-3小时，使研究人员能够在一天内完成从提取的cfDNA到测序的整个流程。NextSeq 550的推荐读长为2 × 149 bp，NextSeq 2000和NovaSeq 6000的推荐读长为2 × 151 bp。
高性能文库制备
Illumina cfDNA Prep with Enrichment可检测低频变异，包括SNV、CNV、插入缺失变异和基因融合。该试剂盒可从仅20 ng cfDNA中识别等位基因频率低至0.2%的小变异，灵敏度≥90%。
从样本到结果的精简工作流程
该试剂盒是集成式工作流程的一部分，从cfDNA提取开始，然后在因美纳中高通量系统上进行测序，并使用DRAGEN for ILMN cfDNA Prep with Enrichment App进行高度准确的变异检出。这种用户友好的解决方案与液体处理自动化兼容，并支持样本拆分，以实现可扩展的效率和高数据质量。
规格
| 系统 | NovaSeq 6000Dx Instrument, NextSeq 550 Desktop Sequencer, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode, NovaSeq X System, NextSeq 500 Desktop Sequencer, NovaSeq 6000 System |
|---|---|
| 方法 | Custom sequencing, Targeted DNA sequencing, Target enrichment |
| 核酸类型 | DNA |
| 特殊的样本类型 | Cell-free DNA |
| 物种 | 人 |
| 技术 | Illumina测序 |
| 变异类别 | Gene fusions, Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) |
需要的产品
完成工作流程需要上述标签板。手动和自动化工作流程有特定的SKU。
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结果
文档
产品资料
支持文档
- Illumina Adapter Sequences
- Illumina cell-free DNA Prep with Enrichment Analysis
- Index Adapters Pooling Guide
- Denature and Dilute Protocol Generator
- DRAGEN Analysis Workflows Product Documentation
- Illumina Cell-Free DNA Prep with Enrichment Product Documentation
- 因美纳接头序列
- Illumina游离DNA Prep with Enrichment分析
- DRAGEN分析工作流程产品
- Illumina Cell-Free DNA Prep with Enrichment产品
- 变性和稀释实验方案生成器
- 标签序列接头混合指南
比较
| Illumina cell-free DNA Prep with Enrichment | Illumina DNA Prep with Enrichment | TruSight Oncology 500 ctDNA v2 | |
|---|---|---|---|
| 系统 | NovaSeq 6000Dx Instrument, NextSeq 550 Desktop Sequencer, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode, NovaSeq X System, NextSeq 500 Desktop Sequencer, NovaSeq 6000 System | MiSeq Desktop Sequencer, iSeq 100 System, NextSeq 550 Desktop Sequencer, NextSeq 2000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System, NovaSeq X Plus System | NovaSeq 6000 System |
| 方法 | Custom sequencing, Targeted DNA sequencing, Target enrichment | Custom sequencing, Targeted DNA sequencing, Exome sequencing, Target enrichment | Targeted DNA sequencing, Target enrichment |
| 核酸类型 | DNA | DNA | DNA |
| 特殊的样本类型 | Cell-free DNA | Blood, Low-input samples, FFPE tissue, Saliva | Circulating tumor DNA, Blood |
| 物种 | 人 | Other, 人 | 人 |
| 技术 | Illumina测序 | Illumina测序 | Illumina测序 |
| 变异类别 | Gene fusions, Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Loss of heterozygosity (LOH), Somatic variants, Germline variants, Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) |
*系统兼容性：对于NextSeq2000，仅测试P3流动槽
Illumina® cfDNA Prep, Ligation (16 Samples)
20104105
Includes ligation-based library prep for preparation of 16 libraries prepared by automation, or 24 libraries prepared manually. Enrichment reagents not included. Analyze data on Illumina Connected Analytics or BaseSpace Sequence Hub for free. Basic subscription to Cloud platform required. Data storage not included. UMI Index Adapters and Unique Dual Indexes are purchased separately and required for library preparation. Choose UMI Index Adapters and Unique Dual Indexes based on automation or manual workflow. Enrichment Panel Content not included.
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Illumina® cfDNA Prep, Ligation (96 Samples)
20104106
Includes ligation-based library prep for preparation of 96 libraries intended for automation, or 120 libraries prepared manually. Enrichment reagents not included. Analyze data on Illumina Connected Analytics or BaseSpace Sequence Hub for free. Basic subscription to Cloud platform required. Data storage not included. UMI Index Adapters and Unique Dual Indexes are purchased separately and required for library preparation. Choose UMI Index Adapters and Unique Dual Indexes based on automation or manual workflow. Enrichment Panel Content not included.
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Illumina® cfDNA Enrichment (16 Reactions)
20104107
Includes fast enrichment reagents for enrichment of Illumina cfDNA Prep libraries for a total of 16 reactions prepared by automation, or 24 reactions prepared manually. Enrichment plexity of 1-plex and 4-plex recommended (pooling of 4 libraries in a single enrichment). Analyze data on Illumina Connected Analytics or BaseSpace Sequence Hub for free. Basic subscription to Cloud platform required. Data storage not included. UMI Index Adapters and Unique Dual Indexes are purchased separately and required for library preparation. Choose UMI Index Adapters and Unique Dual Indexes based on automation or manual workflow. Enrichment Panel Content not included.
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Illumina® cfDNA Prep with Enrichment, Ligation (192 Samples, 4-plex), Cloud Analysis
20104103
Includes ligation-based library prep and fast enrichment reagents for preparation of 192 libraries prepared by automation, or 240 libraries prepared manually, using 4-plex enrichment (pooling of 4 libraries in a single enrichment). Analyze data on Illumina Connected Analytics or BaseSpace Sequence Hub for free. Basic subscription to Cloud platform required. Data storage not included. UMI Index Adapters and Unique Dual Indexes are purchased separately and required for library preparation. Choose UMI Index Adapters and Unique Dual Indexes based on automation or manual workflow. Enrichment Panel Content not included.
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Illumina cfDNA Prep with Enrichment, Ligation (192 Samples, 4-plex) On- premises
20104104
Includes ligation-based library prep and fast enrichment reagents for preparation of 192 libraries prepared by automation, or 240 libraries prepared manually, using 4-plex enrichment (pooling of 4 libraries in a single enrichment). 10,000 Gb, 1 yr DRAGEN Server License included. Sufficient for analyzing sequencing output for up to 400 kb content at 30,000x sequencing depth. Illumina DRAGEN Server required. UMI Index Adapters and Unique Dual Indexes are purchased separately and required for library preparation. Choose UMI Index Adapters and Unique Dual Indexes based on automation or manual workflow. Enrichment Panel Content not included.
配套产品 (4)
IDT® for Illumina® UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)
20034701
UMI DNA Index Anchors（板 = 20031416，盒 = 20032620）+ Nextera Compatible Unique Dual Index A（销售试剂盒 = 20027213，板 = 20025019，盒 = 20026121）
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IDT® for Illumina® UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)
20034702
IDT for Illumina UMI DNA Index Anchors Set B
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IDT for Illumina UMI DNA/DNA Index Anchors Set A for Automation
20066404
包括一盒96个IDT for Illumina - UMI DNA/RNA UD Indexes Set A for Automation，足以标记96个样本，一盒96个IDT for Illumina - UMI DNA Index Anchors for Automation。
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IDT for Illumina UMI DNA/DNA Index Anchors Set B for Automation
20063213
包括一盒96个IDT for Illumina - UMI DNA/RNA UD Indexes Set B for Automation，足以标记96个样本，一盒96个IDT for Illumina - UMI DNA Index Anchors for Automation。
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服务 (1)
Illumina Cell-Free DNA Prep with Enrichment Training
20122119
This 2-day introductory course is designed to familiarize new users with the Illumina cfDNA Prep with Enrichment workflow through detailed hands- on instruction. Participants will learn all essential steps in the workflow, including manual library preparation, enrichment, sequencing, and data analysis.
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